Long-term effects of neonatal capsaicin treatment on intraepidermal nerve fibers and keratinocyte proliferation in rat glabrous skin.

摘要: Innervation is required to preserve several aspects of skin homeostasis. Previous studies in rodents have shown that sciatic nerve transection leads epidermal thinning and reduced keratinocyte proliferation. As the composed sensory motor axons, it not clear whether alterations reflect or disturbances. In this study, we used neonatal capsaicin treatment evaluate chemical denervation affects proliferation at 1, 3, 6 months age. Using design-based stereological methods, estimated total length intraepidermal fibers (IENF) were peptidergic type number bromodeoxyuridine-labeled (BrdU+) nuclei hind paw glabrous epidermis control capsaicin-treated rats. We found decreased fiber IENF immunoreactive for both protein gene product 9.5 (PGP+) calcitonin gene-related peptide (CGRP+). The PGP+ by 83%, 81%, 77% CGRP+ 48%, 58%, 58% months, respectively. Double-immunofluorescence staining neural beta III tubulin CGRP revealed majority remaining after type. BrdU+ was similar groups. Our findings suggest present are sufficient maintain replacement. Anat Rec, 2010. © 2010 Wiley-Liss, Inc.