Quantifying inbreeding in natural populations of hermaphroditic organisms

摘要: We review molecular methods for estimating selfing rates and inbreeding in populations. Two main approaches are available: the population structure approach (PSA) progeny-array (PAA). The PSA relies on single-generation samples produces estimates that integrate history over several generations, but is based strong assumptions (for example, equilibrium). has classically relied single-locus coefficients averaged loci. Unfortunately very sensitive to technical problems such as occurrence of null alleles at one or more Consequently might be substantially overestimated, especially outbred However, robustness recently been greatly improved by development multilocus free bias. PAA, other hand, comparison between offspring mother genotypes. As a consequence, PAA do not reflect long-term only recent mating events maternal individuals studied (‘here now’ selfing). In addition rates, allows system parameters, including biparental correlation among sibs. Although could also biased problems, incompatibilities mother's genotype her allow identification correction For all methods, we provide guidelines required number loci sample sizes. conclude equally robust, provided information used. experimental constraints may make demanding, animals, two complementary information, can fruitfully conducted together.