Crystallization with hapten of the Fab' fragment from a mouse IgA myeloma protein with antidinitrophenyl activity.
作者： D. Inbar , M. Rotman , David Givol
DOI: 10.1016/S0021-9258(18)61784-3
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摘要: Abstract Protein 315, a mouse myeloma IgA protein which binds nitrophenyl ligands, and its pepsin-produced Fab' fragment have been purified by affinity chromatography. The fragments were found to be homogeneous polyacrylamide electrophoresis isoelectric focusing, possess uniform binding constant. These readily crystallizable at low salt concentration near their point (pH 4.7). Similarly crystals can obtained of the Fab'-hapten complex. Such 1:1 molar ratio hapten (e, N-dinitrophenyl lysine) protein.
sci-hub.st 参考文章(8)
Osserman Ef, Fahey Jl, Terry Wd, Kunkel Hg, Franklin Ec, Notation for human immunogobulin subclasses. Bulletin of The World Health Organization. ,vol. 35, pp. 953- 953 ,(1966)
Guido Rossi, Alfred Nisonoff, Crystallization of Fragment Fab of human IgG myeloma proteins Biochemical and Biophysical Research Communications. ,vol. 31, pp. 914- 918 ,(1968) , 10.1016/0006-291X(68)90539-1
Edward J. Goetzl, Henry Metzger, Affinity labeling of a mouse myeloma protein which binds nitrophenyl ligands. Kinetics of labeling and isolation of a labeled peptide. Biochemistry. ,vol. 9, pp. 1267- 1278 ,(1970) , 10.1021/BI00807A031
Herman N. Eisen, Ernest S. Simms, Michael. Potter, Mouse myeloma proteins with antihapten antibody activity. Protein produced by plasma cell tumor MOPC-315 Biochemistry. ,vol. 7, pp. 4126- 4134 ,(1968) , 10.1021/BI00851A048
W. D. TERRY, B. W. MATTHEWS, D. R. DAVIES, Crystallographic studies of a human immunoglobulin. Nature. ,vol. 220, pp. 239- 241 ,(1968) , 10.1038/220239A0
R.L. Humphrey, H.P. Avey, L.N. Becka, R.J. Poljak, G. Rossi, T.K. Choi, A. Nisonoff, X-ray crystallographic study of the fab fragments from two human myeloma proteins Journal of Molecular Biology. ,vol. 43, pp. 223- 226 ,(1969) , 10.1016/0022-2836(69)90092-8
G. ROSSI, T. K. CHOI, A. NISONOFF, Crystals of fragment Fab': preparation from pepsin digests of human IgG myeloma proteins. Nature. ,vol. 223, pp. 837- 838 ,(1969) , 10.1038/223837A0
Baruch J. Davis, DISC ELECTROPHORESIS - II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS* Annals of the New York Academy of Sciences. ,vol. 121, pp. 404- 427 ,(2006) , 10.1111/J.1749-6632.1964.TB14213.X