The cooperative roles of PHO80-like cyclins in regulating the G1/S transition and posterior cytoskeletal morphogenesis in Trypanosoma brucei.

摘要: Summary Cyclins and cyclin-dependent kinases (CDKs) represent the fundamental, crucial regulators of cell division cycle in eukaryotes. Trypanosoma brucei expresses a large number cyclins Cdc2-related (CRKs). However, how these CRKs cooperate to regulate progression remains poorly understood. Here, we carry out directional yeast two-hybrid assays identify interactions between 10 11 detect total 26 cyclin–CRK pairs, among which 20 pairs are new. Our current efforts focused on four PHO80-like cyclins, CYC2, CYC4, CYC5 CYC7, their physical functional with CRK1. Silencing CRK1 leads increase G1 cells defective DNA replication, suggesting important roles promoting G1/S transition. Additionally, CYC2-, CYC7- CRK1-deficient possess an elongated posterior that is filled newly assembled microtubules. Further, show display distinct subcellular localizations half-lives, they likely undergo regulation. Altogether, our results demonstrate involvement CRK1-associated transition requirement CYC2 CYC7 maintaining cytoskeletal morphogenesis during