Transcriptional signature of human macrophages exposed to the environmental contaminant benzo(a)pyrene
作者： Lydie Sparfel , Marie-Laure Pinel-Marie , Magali Boize , Serge Koscielny , Sophie Desmots
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摘要: Polycyclic aromatic hydrocarbons (PAHs) are widely distributed immunotoxic and carcinogenic environmental contaminants, known to affect macrophages. In order identify their molecular targets in such cells, we have analyzed gene expression profile of primary human macrophages treated by the prototypical PAH benzo(a)pyrene (BaP), using pangenomic oligonucleotides microarrays. Exposure BaP for 8 24 h resulted 96 1100 genes, differentially expressed at least a twofold change factor, respectively. Some these targets, including chemokine receptor CXCR5, G protein-coupled 35 (GPR35), Ras regulator RASAL1, not been previously shown be affected PAHs, contrast others, as interleukin-1beta aryl hydrocarbon (AhR) repressor. These BaP-mediated regulations were fully validated reverse transcription-quantitative polymerase chain reaction assays some selected genes. Their bioinformatic analysis indicated that biological functions linked immunity, inflammation, cell death among most AhR p53 signaling pathways significant canonical activated PAH. implications moreover confirmed prevention BaP-related upregulation target genes silencing or use pifithrin-alpha, an inhibitor bioactivation-related DNA damage/p53 pathways. Overall, data, through identifying targeted PAHs macrophages, may contribute better understand basis immunotoxicity contaminants.
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John H. Kehrl, Il-Young Hwang, Chung Park, Chemoattract receptor signaling and its role in lymphocyte motility and trafficking. Current Topics in Microbiology and Immunology. ,vol. 334, pp. 107- 127 ,(2009) , 10.1007/978-3-540-93864-4_5
Sarah L Hockley, Volker M Arlt, Daniel Brewer, Ian Giddings, David H Phillips, Time- and concentration-dependent changes in gene expression induced by benzo(a)pyrene in two human cell lines, MCF-7 and HepG2 BMC Genomics. ,vol. 7, pp. 260- 260 ,(2006) , 10.1186/1471-2164-7-260
Jinghong Wang, Nicole Simonavicius, Xiaosu Wu, Gayathri Swaminath, Jeff Reagan, Hui Tian, Lei Ling, Kynurenic Acid as a Ligand for Orphan G Protein-coupled Receptor GPR35 Journal of Biological Chemistry. ,vol. 281, pp. 22021- 22028 ,(2006) , 10.1074/JBC.M603503200
Patricia Monteiro, David Gilot, Eric Le Ferrec, Valérie Lecureur, Monique N’diaye, Marc Le Vee, Normand Podechard, Celio Pouponnot, Olivier Fardel, AhR- and c-maf-dependent induction of β7-integrin expression in human macrophages in response to environmental polycyclic aromatic hydrocarbons Biochemical and Biophysical Research Communications. ,vol. 358, pp. 442- 448 ,(2007) , 10.1016/J.BBRC.2007.04.111
Christoph F.A. Vogel, Samuel R. Goth, Bin Dong, Isaac N. Pessah, Fumio Matsumura, Aryl hydrocarbon receptor signaling mediates expression of indoleamine 2,3-dioxygenase Biochemical and Biophysical Research Communications. ,vol. 375, pp. 331- 335 ,(2008) , 10.1016/J.BBRC.2008.07.156
Xiangyun Lu, Jimin Shao, Hongjuan Li, Yingnian Yu, Early whole-genome transcriptional response induced by benzo[a]pyrene diol epoxide in a normal human cell line. Genomics. ,vol. 93, pp. 332- 342 ,(2009) , 10.1016/J.YGENO.2008.12.007
B. H. Chen, Y. S. Lin, Formation of Polycyclic Aromatic Hydrocarbons during Processing of Duck Meat Journal of Agricultural and Food Chemistry. ,vol. 45, pp. 1394- 1403 ,(1997) , 10.1021/JF9606363
W.J. de Waard, J.M.M.J.G. Aarts, A.A.C.M. Peijnenburg, H. Baykus, E. Talsma, A. Punt, T.M.C.M. de Kok, F.J. van Schooten, L.A.P. Hoogenboom, Gene expression profiling in Caco-2 human colon cells exposed to TCDD, benzo[a]pyrene, and natural Ah receptor agonists from cruciferous vegetables and citrus fruits Toxicology in Vitro. ,vol. 22, pp. 396- 410 ,(2008) , 10.1016/J.TIV.2007.10.007
Leming Shi, Laura H Reid, Wendell D Jones, Richard Shippy, Janet A Warrington, Shawn C Baker, Patrick J Collins, Francoise De Longueville, Ernest S Kawasaki, Kathleen Y Lee, Yuling Luo, Yongming Andrew Sun, James C Willey, Robert A Setterquist, Gavin M Fischer, Weida Tong, Yvonne P Dragan, David J Dix, Felix W Frueh, Federico M Goodsaid, Damir Herman, Roderick V Jensen, Charles D Johnson, Edward K Lobenhofer, Raj K Puri, Uwe Schrf, Jean Thierry-Mieg, Charles Wang, Mike Wilson, Paul K Wolber, Lu Zhang, Shashi Amur, Wenjun Bao, Catalin C Barbacioru, Anne Bergstrom Lucas, Vincent Bertholet, Cecilie Boysen, Bud Bromley, Donna Brown, Alan Brunner, Roger Canales, Xiaoxi Megan Cao, Thomas A Cebula, James J Chen, Jing Cheng, Tzu-Ming Chu, Eugene Chudin, John Corson, J Christopher Corton, Lisa J Croner, Christopher Davies, Timothy S Davison, Glenda Delenstarr, Xutao Deng, David Dorris, Aron C Eklund, Xiao-Hui Fan, Hong Fang, Stephanie Fulmer-Smentek, James C Fuscoe, Kathryn Gallagher, Weigong Ge, Lei Guo, Xu Guo, Janet Hager, Paul K Haje, Jing Han, Tao Han, Heather C Harbottle, Stephen C Harris, Eli Hatchwell, Craig A Hauser, Susan Hester, Huixiao Hong, Patrick Hurban, Scott A Jackson, Hanlee Ji, Charles R Knight, Winston P Kuo, J Eugene LeClerc, Shawn Levy, Quan-Zhen Li, Chunmei Liu, Ying Liu, Michael J Lombardi, Yunqing Ma, Scott R Magnuson, Botoul Maqsodi, Tim McDaniel, Nan Mei, Ola Myklebost, Baitang Ning, Natalia Novoradovskaya, Michael S Orr, Terry W Osborn, Adam Papallo, Tucker A Patterson, Roger G Perkins, Elizabeth H Peters, Ron Peterson, Kenneth L Philips, P Scott Pine, Lajos Pusztai, Feng Qian, Hongzu Ren, Mitch Rosen, Barry A Rosenzweig, Raymond R Samaha, Mark Schena, Gary P Schroth, Svetlana Shchegrova, Dave D Smith, Frank Staedtler, Zhenqiang Su, Hongmei Sun, Zoltan Szallasi, Zivana Tezak, Danielle Thierry-Mieg, Karol L Thompson, Irina Tikhonova, Yaron Turpaz, Beena Vallanat, Christophe Van, Stephen J Walker, Sue Jane Wang, Yonghong Wang, Russ Wolfinger, Alex Wong, Jie Wu, Chunlin Xiao, Qian Xie, Jun Xu, Wen Yang, S Zhong, Y Zong, W Slikker Jr, None, The MicroArray Quality Control (MAQC) project shows inter- and intraplatform reproducibility of gene expression measurements Nature Biotechnology. ,vol. 24, pp. 1151- 1161 ,(2006) , 10.1038/NBT1239
Marie-Laure Pinel-Marie, Lydie Sparfel, Sophie Desmots, Olivier Fardel, Aryl hydrocarbon receptor-dependent induction of the NADPH oxidase subunit NCF1/p47phox expression leading to priming of human macrophage oxidative burst Free Radical Biology and Medicine. ,vol. 47, pp. 825- 834 ,(2009) , 10.1016/J.FREERADBIOMED.2009.06.025