A microfabrication-based dynamic array cytometer.

摘要: We have developed a microfabricated device for use in parallel luminescent single-cell assays that can sort populations upon the basis of dynamic functional responses to stimuli. This is composed regular array noncontact traps. These traps dielectrophoresis stably confine cells and hold them against disrupting fluid flows. Using quantitative modeling, we designed with novel asymmetric extruded-quadrupole geometry. new trap be physically arrayed electrically addressed, enabling our cytometer. Situating an these microchannel, introduced into demonstrated observation fluorescent followed by sorting. Such has potential investigating processes, as revealed temporal behavior, large numbers single cells.