Pathogenesis-Related Gene, JcPR-10a from Jatropha curcas Exhibit RNase and Antifungal Activity

摘要: The pathogenesis-related proteins have a broad spectrum of roles, ranging from seed germination, development to resistance. PR-10 is multigene family differing other PR in being intracellular, small and acidic with similar 3D structures. We isolated JcPR-10a cDNA an ORF 483 bp J. curcas, important biofuel crop grown the wastelands India. gets clustered dicots phylogenetic tree. genomic organisation analysis revealed presence intron at conserved 185 position. Transcript expression was upregulated response different stimuli such as NaCl, salicylic acid, methyl jasmonate M. phaseolina. In SA Macrophomina transcript found increased 48 h, however, case NaCl MeJa strong induction observed 12 h which decreased h. first time report up regulation gene by Macrophomina, pathogen causing collar rot Jatropha. recombinant E. coli cells showed better growth LB medium supplemented whereas inhibited KCl, mannitol, sorbitol, acid. protein overexpressed cells, purified homogeneity, exhibited RNase DNase activity. Furthermore, also antifungal activity against indicating that can serve candidate engineer stress tolerance Jatropha well plants susceptible Macrophomina.