A β‐subclass phosphatidylinositol‐specific phospholipase C from squid (Loligo forbesi) photoreceptors exhibiting a truncated C‐terminus
作者： Alan Carne , Richard A. McGregor , Jeetendra Bhatia , Asipu Sivaprasadarao , Jeff N. Keen
DOI: 10.1016/0014-5793(95)00936-4
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摘要: Abstract A PCR-based strategy has been used to isolate a full length cDNA encoding phosphatidylinositol-specific phospholipase C from sized squid ( Loligo forbesi ) retinal library. The predicted protein sequence contains 875 amino acids, with calculated M r 98,181, and marked similarity PLC β-isoforms, including conservation of the ‘X’ ‘Y’ regions. It is unique in having major C-terminal truncation. apparent 120,000 estimated by SDS-PAGE isolated photoreceptors identified partial analysis correspond clone. This shown hydrolyse phosphatidylinositol 4,5-bisphosphate. not yet clear whether this represents light-activated vision.
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