Identification of water-deficit responsive genes in maritime pine (Pinus pinaster Ait.) roots.

摘要: Root adaptation to soil environmental factors is very important maritime pine, the main conifer species used for reforestation in France. The range of climates sites where this established varies from flooded winter drought-prone summer. No studies have yet focused on morphological, physiological or molecular variability root system adapt its growth such an environment. We developed a strategy isolate drought-responsive genes tissue order identify mechanisms that trees evolved cope with drought (the problem affecting wood productivity), and exploit information improve stress tolerance. In provide easy access system, seedlings were raised hydroponic solution. Polyethylene glycol was as osmoticum induce water deficit. Using cDNA-AFLP technique, we screened more than 2500 transcript derived fragments, which 33 (1.2%) showed clear variation presence/absence between non stressed medium. relative abundance these transcripts then analysed by reverse northern. Only two out significant opposite behaviour both techniques. identification characterization water-deficit responsive roots emergence understanding patterns gene expression regulation involved response pine.