Aldehyde oxidase assay by capillary electrophoresis: From off-line, online up to immobilized enzyme reactor.

摘要: Capillary electrophoresis is a modern separation technique characterized by many benefits, which qualify it also for enzyme assays and the study of kinetics during drug development. Homogeneous or heterogeneous approaches can be followed enzymatic incubation. In this study, an immobilization procedure aldehyde oxidase on magnetic particles was developed considering their integration with capillary electrophoresis. A number nano/microparticle types were tested purpose, showing that most active when immobilized bare silica nanoparticles. Primarily, reusability nanoparticles tested. Three consecutive incubations substrate could performed, but activity considerably dropped after first One reason detachment from nanoparticles, no release detected neither at 4°C nor 37°C 5 h. The drop in observed incubations, due to inactivation over time given temperature. For stored 4°C, decreased 83% h, contrast steep decrease 37%.