Characterization of unique lymphoid cells derived from murine spleen which constitutively produce interleukin-6.

摘要: Attempts have been made to isolate continuous lines of rare subsets lymphoid cells present in murine spleen order analyse their function and lineage relationship with respect other cells. Mitogenic stimulation was used expand the remaining following depletion CD4+ CD8+ T by antibody complement treatment. Cells were cultured presence concanavalin A (Con A), interleukin-2 (IL-2) syngeneic irradiated feeder This procedure expanded a population non-T-, non-B-lymphoid bearing common, unique phenotype resembling precursors. Eight cloned from B10.A(2R) B10.A(5R) strains mice analysed here. Analysis cell surface marker expression has revealed positive class I II major histocompatibility complex (MHC) antigens, CD44, CD45 (T200 B220) but expressing no markers T, B or myeloid All represent agranular lymphoblasts show evidence early T-cell receptor (TcR) Ig heavy chain gene rearrangements, suggesting commitment T-or B-lymphoid lineage. Despite NK1.1 for natural killer (NK) cells, none shown cytotoxic NK targets, nor could be induced various activation procedures. lymphokine production detectable IL-1, IL-2, IL-3, IL-4, IL-5, tumour necrosis factor-alpha (TNF-alpha) granulocyte-macrophage colony-stimulating factor (GM-CSF) supernatants. However, all one these constitutively produce IL-6. Each line induce proliferation independently mixed lymphocyte reactions, implicating capacity act as antigen-presenting Consistent this hypothesis is observation that also demonstrate endocytic activity foreign proteins. visualized uptake fluoresceinated albumin into cytoplasmic granules. Since they express many common described isolates dendritic including both molecules, would appear first example subset.