Double jeopardy: both overexpression and suppression of a redox-activated plant mitogen-activated protein kinase render tobacco plants ozone sensitive.

摘要: In plants, the role of mitogen-activated protein kinase (MAPK) in reactive oxygen species (ROS)-based signal transduction processes is elusive. Despite fact that ROS can induce MAPK activation, no direct genetic evidence has linked ROS-induced activation with hypersensitive response, a form programmed cell death. tobacco, major salicylate-induced (SIPK). We found through gain-of-function and loss-of-function approaches both overexpression RNA interference-based suppression SIPK render plant sensitive to stress. Transgenic lines overexpressing nonphosphorylatable version were not sensitive. Analysis profiles ROS-stressed transgenic wild-type plants revealed striking interplay between another (wound-induced [WIPK]) different kinotypes. During continuous ozone exposure, abnormally prolonged was seen SIPK-overexpression genotype, without WIPK whereas strong stable observed SIPK-suppressed lines. Thus, one activated tobacco cells upon stimulation appears be control inactivation WIPK.