Antigenic and antiheparin properties of human platelet factor 4 (PF4)
作者： N Nath , CT Lowery , S Niewiarowski
DOI: 10.1182/BLOOD.V45.4.537.537
关键词:
摘要: Platelet factor 4 (PF4, a heparin-neutralizing protein) was isolated from washed human platelets. It found to be homogenous by SDS- polyacrylamide gel electrophoresis, immunodiffusion, and immunoelectrophoresis, when tested with monospecific antibody produced in rabbits. PF4 is heat-stable protein, but its antiheparin activity antigenicity are destroyed trypsin. The molecular weight of as calculated amino acid analysis approximately 8000 electrophoresis beta-mercaptoethanol, 7100 daltons. migrated the cathode at pH 8.6. interaction heparin resulted formation complex which anode, immunoelectrophoresis. Incubation purified 37 degrees C loss activity. presence PG4 antigen material released during platelet aggregation various agents stages preparative procedure closely correlated. has been concluded that two properties same protein. Comparison pig revealed significant biochemical antigenic differences.
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