Omega-3 Polyunsaturated Fatty Acids Prevent Toxoplasma gondii Infection by Inducing Autophagy via AMPK Activation.

摘要: Omega-3 polyunsaturated fatty acids (ω3-PUFAs) have potential protective activity in a variety of infectious diseases, but their actions and underlying mechanisms Toxoplasma gondii infection remain poorly understood. Here, we report that docosahexaenoic acid (DHA) robustly induced autophagy murine bone marrow-derived macrophages (BMDMs). Treatment T. gondii-infected with DHA resulted colocalization parasitophorous vacuoles autophagosomes reduced intracellular survival gondii. The autophagic anti-Toxoplasma effects by were mediated AMP-activated protein kinase (AMPK) signaling. Importantly, BMDMs isolated from Fat-1 transgenic mice, well-known animal model capable synthesizing ω3-PUFAs ω6-PUFAs, showed increased activation AMPK, leading to when compared wild-type BMDMs. Moreover, mice exhibited lower cyst burden the brain following avirulent strain ME49 than mice. Collectively, our results revealed which endogenous control suggest might serve as therapeutic candidate prevent toxoplasmosis other protozoan parasites.