Extracellular matrix (mesoglea) of Hydra vulgaris III. Formation and function during morphogenesis of hydra cell aggregates.

摘要: Hydra, as a member of the phylum Cnidaria, is characterized by body lining organized an epithelial bilayer with intervening extracellular matrix (ECM) termed mesoglea. Previous studies have established that mesoglea has components indicative mammalian ECM such type IV collagen, laminin, fibronectin, and heparan sulfate proteoglycan, these appear to play critical role in hydra head regeneration. A remarkable feature its ability reorganize into adult structure within 96 hr 7 days from pellets formed dissociated cells. This regenerative model been cell aggregate system. The present study designed characterize biogenesis aggregates determine morphogenesis aggregates. We find first form 12 development then subsequently develop Morphogenesis follows formation Immunofluorescence indicate are deposited between about 12-17 pellet formation, pulse-labeling translation rate peaks 48-72 development. Ultrastructural mature 48-96 formation. Drugs β-aminoproprionitrile 2,2′-dipydridyl, which interfere cross-linking collagens, p -nitrophenyl-β-d-xylopyranoside, interferes addition GAG moieties proteoglycan core molecules, were found reversibly block Transmission electron microscopy drugs affect ultrastructure In addition, both polyclonal monoclonal antibodies raised isolated These (1) rapid precedes morphogenetic processes during development, (2) essential for normal