Isolation and Initial Characterization of Resistant Cells to Photodynamic Therapy

摘要: After photodynamic therapy (PDT), the apparition of resistant tumor cells can occur. Laboratory models are being developed in order to understand potential mechanisms implicated such resistance. In this sense, we describe methods published for isolation and characterization PDT. We also propose other unpublished procedures that could be interest study Factors as parental cell line, photosensitizer (PS) (or prodrug), treatment conditions, interval, clonal or total population selection have taken into consideration. Treatment doses generally high repeated over time. The development PDT take several months. populations vs performed by using different cellular molecular techniques, including: morphology analysis, intracellular PS content measurement, localization, migration invasion capacity, expression distribution adhesion proteins, death proteins evaluation specific genes proliferation survival. Transplantation mouse contribute determine biological activity PDT-resistant vivo, allowing their tumorigenicity aggressiveness. will help us how resistance anticancer affects functional aspects vitro which necessary improve clinical results.