Identification of hub genes and pathways associated with bladder cancer based on co-expression network analysis

摘要: The aim of the present study was to identify hub genes and signaling pathways associated with bladder cancer (BC) utilizing centrality analysis pathway enrichment analysis. differentially expressed (DEGs) were screened from ArrayExpress database between normal subjects BC patients. Co-expression networks constructed using co-expressed links, investigated by degree co-expression in BC. enriched Kyoto Encyclopedia Genes Genomes based on DEGs. gene expression tissues validated reverse transcription-quantitative polymerase chain reaction (RT-qPCR) western blotting. A total 329 DEGs screened, including 147 upregulated 182 downregulated genes. network controls consisted nodes 434 edges, two each pair Centrality suggested that top 5 high included lectin, galactoside-binding, soluble, 4 (LGALS4), protein tyrosine phosphatase, receptor type N2 (PTPRN2), transmembrane protease, serine 11E (TMPRSS11E), tripartite motif containing 31 (TRIM31) potassium voltage-gated channel subfamily D member 3 (KCND3). Pathway revealed significantly terms (cell cycle, DNA replication, oocyte meiosis, p53 peroxisome proliferator-activated pathway). According RT-qPCR blot analysis, 4/5 expressed, LGALS4, PTPRN2, TMPRSS11E, TRIM31; however, KCND3 not expressed. In study, successfully identified (LGALS4, TRIM31 KCND3) biological may be underlying biomarkers for early diagnosis treatment revealed.