Human DNA methyltransferase gene DNMT1 is regulated by the APC pathway.
作者： Paul M Campbell , Moshe Szyf
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摘要: Epigenomic changes in DNA methylation patterns are evident a variety of cancers, including colorectal cancer (CRC). In addition, large proportion CRC tumors and cell lines harbor genetic mutations the APC/beta-catenin/TCF transcription activation pathway. While several target genes have been proposed, causal downstream agent between APC mutation has not fully established. Because previous work implicates methyltransferase (DMNT1) as critical point tumorigenesis recent studies suggest that familial also exhibits epigenetic alterations, we sought to investigate whether this gene might be regulated by CRC. Reconstitution wild type HT-29 reduced expression both reporter driven minimal DNMT1 promoter mRNA is independent growth stasis. We provide evidence for role demonstrating antisense-driven reduction inhibits anchorage-independent growth, an indicator tumorigenesis, cells. These data support future consideration treatment
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