An essential sensor histidine kinase controlled by transmembrane helix interactions with its auxiliary proteins

摘要: Two-component signal transduction systems with membrane-embedded sensor histidine kinases are believed to recognize environmental signals and transduce this information over the cellular membrane influence activity of a transcription factor which they mated. The YycG kinase Bacillus subtilis, containing two transmembrane helices, is subject complicated activity-control circuit involving other proteins N-terminal YycH YycI. Truncation studies YycI demonstrated that individual helices these sufficient adjust activity, indicating control achieved at level. A replica exchange molecular dynamics computational approach generated in silico structural models helix complex informed mutagenesis supporting accuracy model. results predict recognition by any extracellular domains or associated transmitted across subtle alterations positions within three proteins.