Hydrostatic pressure-dependent changes in cyclic AMP signaling in optic nerve head astrocytes from Caucasian and African American donors.

摘要: PURPOSE Investigate the effect of hydrostatic pressure (HP) on 3', 5'-cyclic adenosine monophosphate (cAMP) levels and downstream signaling in cultures normal optic nerve head (ONH) astrocytes from Caucasian American (CA) African (AA) donors. METHODS Intracellular cAMP were assayed after exposing ONH to HP for varying times. Quantitative RT-PCR was used determine expression selected pathway genes human treatment. Western blots measure changes phosphorylation state response element binding protein (CREB) subjected HP, ATP, phosphodiesterase or kinase inhibitors. RESULTS The basal intracellular level is similar among AA CA astrocytes. After exposure 15 min 30 presence a inhibitor further increase observed astrocytes, but not Consistent with activation cAMP-dependent pathway, CREB (Ser-133) increased greater extent than 3 h HP. Exposure elevated 3-6 differentially altered (ADCY3, ADCY9, PTHLH, PDE7B) compared Treatment ATP more suggesting differential Ca(2+) these populations. CONCLUSIONS Activation by may be an important contributor susceptibility intraocular glaucoma AA, population at higher risk disease.