Dissociated limb bud cells of chick embryos can express lens specificity when reaggregated and cultured in vitro
作者： Ryuji Kodama , Goro Eguchi
DOI: 10.1016/0012-1606(82)90027-6
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摘要: Abstract Limb bud cells of chick embryos (stages 23–24) were dissociated into single cells, reaggregated, and cultured in vitro for about a week. δ-Crystallin, generally thought to be lens-specific protein the chick, was detected aggregates by indirect immunofluorescent staining, double immunodiffusion test, immunoelectrophoresis with specific antiserum against δ-crystallin. Cells containing δ-crystallin distributed epidermal cell clusters also mesenchymal tissues surrounding cartilage nodules aggregates. Those shown have originated from mesoderm limb bud, those probably ectoderm. The possible cellular origin this appearance discussed.
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