Transcriptome analysis of Drosophila CNS midline cells reveals diverse peptidergic properties and a role for castor in neuronal differentiation

摘要: One of the key aspects neuronal differentiation is array neurotransmitters and neurotransmitter receptors that each neuron possesses. important goal developmental neuroscience to understand how these differentiated properties are established during development. In this paper, we use fluorescence activated cell sorting RNA-seq determine transcriptome Drosophila CNS midline cells, which consist a small number well-characterized neurons glia. These data revealed cells express 9 neuropeptide precursor genes, 13 receptor 31 small-molecule genes. situ hybridization high-resolution confocal analyses were carried-out identity for neuropeptides receptors. The results surprising level diversity. Neuropeptide genes expressed in variety types, including motoneurons, GABAergic interneurons, previously unknown functional differences among highly-related iVUM neurons. There also exist segmental expression same sub-type. Similar experiments on midline-expressed reveal considerable diversity synaptic inputs. Multiple types interneurons and, one case, link feeding behavior gut peristalsis locomotion. differences, variations between 3 iVUMs, three hormone broadly most cells. Castor transcription factor present at high levels iVUM5, both expresses short F gene. Genetic misexpression indicated castor specifically controls gene, but does not affect fate or Gad1. This indicates novel function regulating gene expression.