Evidence for trehalose‐6‐phosphate‐dependent and ‐independent mechanisms in the control of sugar influx into yeast glycolysis

摘要: In the yeast Saccharomyces cerevisiae, trehalose-6-phosphate (tre-6-P) synthase encoded by GGS1/TPS1, is not only involved in production of trehalose but also restriction sugar influx into glycolysis an unknown fashion; it therefore essential for growth on glucose or fructose. this work, we have deleted TPS2 gene encoding tre-6-P phosphatase a strain which displays very low levels Ggs1/TPS1, as result presence byp 1-3 allele GGS1/TPS1. The tps2 delta double mutant showed elevated along with improved and ethanol production, although estimated concentrations glycolytic metabolites indicated excessive influx. wild-type strain, addition caused rapid transient increase tre-6-P. tps 2 cells, high level before addition, appeared to be diminished. Furthermore, confirmed that inhibits hexokinases vitro. These data are consistent through inhibition during switch fermentative metabolism. During logarithmic cells was lower than mutant. However, latter arrested after about four generations. Hence, other mechanisms, depend Ggs1/Tps1, appear control glucose. provide evidence requirement Ggs1/Tps1 sporulation may unrelated its involvement metabolism system controlling glycolysis.