Dendrotoxin acceptor from bovine synaptic plasma membranes. Binding properties, purification and subunit composition of a putative constituent of certain voltage-activated K+ channels.

摘要: Dendrotoxin is a snake polypeptide that blocks selectively and potently certain voltage-sensitive, fast-activating K+ channels in the nervous system, where it binds with high affinity to membranous acceptors. Herein, acceptor protein for dendrotoxin bovine synaptic membranes solubilized active form its complete purification achieved by chromatography, involving novel elution procedure. This putative K+-channel constituent shown be large oligomeric glycoprotein containing two major subunits, Mr values of 75,000 37,000.