Alloreactivity as a Source of High Avidity Peptide-Specific Human CTL

摘要: PBL from HLA-A2− or HLA-A3− donors were stimulated with synthetic peptide libraries fitting HLA-A2 HLA-A3 motifs and presented on HLA-A2- HLA-A3-expressing TAP− cells. Peptide library-specific allorestricted CTL found to constitute up half the alloreactive response occurred at twofold lower frequency than autologous CTL. This indicates that positive selection by one particular MHC class I molecule is not absolutely essential for generation of restricted same molecule. However, increases their frequency. The obtained differed greatly both respect dependency specificity. Determination avidity representative clone, 10F4, proved method described here allows stimulation high cytotoxic T approach involving in vitro cells toward a was present during negative might therefore offer possibility isolating against self foreign peptides varying avidities. Such indeed be useful tumor immunotherapy.