Non-destructive real-time imaging of cell seeded tissue engineered scaffolds

摘要: The use of tissue engineered scaffolds in combination with progenitor cells has emerged as a promising strategy to restore or replace tissues damaged by disease trauma. In addition being biocompatible and exhibiting appropriate mechanical properties, must be designed sustain cell attachment, proliferation, differentiation ultimately produce the desired once implanted patient [1]. Conventional techniques used assess successful scaffold design include viability stains, DNA assays, histological sectioning/staining. While significant information can gained from using these methodologies, they are destructive sample only provide snapshots development at limited number time points. Consequently, key temporal spatial relating regeneration is lost utilizing techniques. Thus, ability non-destructively monitor viability, real-time great importance for engineering [2].Copyright © 2011 ASME