Imaging phluorin-based probes at hippocampal synapses.
作者： Stephen J. Royle , Björn Granseth , Benjamin Odermatt , Aude Derevier , Leon Lagnado
DOI: 10.1007/978-1-59745-261-8_22
关键词:
摘要: Accurate measurement of synaptic vesicle exocytosis and endocytosis is crucial to understanding the molecular basis transmission. The fusion a pH-sensitive green fluorescent protein (pHluorin) various proteins has allowed study recycling in real time. Two such probes, synaptopHluorin sypHy, have been imaged at synapses hippocampal neurons culture. combination these reporters with techniques for interference, as RNAi allows molecules involved recycling. Here authors describe methods culture transfection neurons, imaging pHluorin-based probes analysis pHluorin signals down resolution individual vesicles.
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