High-resolution in situ hybridization histochemistry.
作者： Frank Baldino , Marie-Francoise Chesselet , Michael E. Lewis
DOI: 10.1016/0076-6879(89)68057-3
关键词:
摘要: Publisher Summary This chapter describes how to perform in situ hybridization histochemistry (ISHH) nervous tissue with the high degree of resolution required assay these hybrids within cytoplasm individual neurons. Oligonucleotide and RNA probes are most commonly used for ISHH. Each type probe has specific advantages but cases, a can be similar success. Several aspects ISHH procedure common both types described. Application technology neurobiology already yielded insights into cellular mechanisms mediating plasticity messenger (mRNA) expression induced by humoral synaptic manipulation central system (CNS) neuroendocrine tissues. The detection intron sequences heteronuclear mRNA species permits, first time, evaluation rate transcription at level single cell. Thus, high-resolution provides histochemical technique study molecular basis genomic plasticity.
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