A piezoelectric method for monitoring formaldehyde induced crosslink formation between poly-lysine and poly-deoxyguanosine

摘要: Abstract To date, no experimental technique has been used to monitor DNA-protein crosslink formation in real-time. Real-time data is important for understanding the underlying chemical mechanisms associated with this reaction process. Here, novel adaptation of existing piezoelectric quartz crystal (PQC) or microbalance (QCM) technology was monitor, real-time, a bond induced by formaldehyde between lysine and guanine. Previous results showed complexes guanine constitute major portion crosslinks formed. Thus, poly-lysine5 poly-deoxyguanosine11 were as model system develop detection method. Poly-lysine5 immobilized on QCM electrode surfaces covalent attachment through polyethylenimine (PEI). Immobilization confirmed decrease dry frequency; consistency suggested uniform coatings produced. The sensor configured within thermostatic environmental chamber. calibrated baseline responses variations analyte solution matrix identified. QCMs placed contact poly-deoxyguanosine11, monitored Crosslink verified evaluation controls. Control assays indicated some frequency signal aresult non-specific association. Further conducted after saturation binding. This real-time represents significant advancement state knowledge crosslinking process provides foundation further investigations.