Profiling of Active Microorganisms by Stable Isotope Probing-Metagenomics
作者： Eileen Kröber , Özge Eyice
DOI: 10.1007/978-1-4939-9721-3_12
关键词:
摘要: Stable isotope probing (SIP) provides researchers a culture-independent method to retrieve nucleic acids from active microbial populations performing specific metabolic activity in complex ecosystems. In recent years, the use of SIP ecology studies has been accelerated. This is partly due advances sequencing and bioinformatics tools, which enable fast reliable analysis DNA RNA experiments. One these metagenomics, contributed significantly body knowledge by providing data not only on taxonomy but also key functional genes pathways their relative abundances. this chapter, we provide general background application SIP-metagenomics approach workflow for metagenomic datasets using most up-to-date tools.
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