Functional-genomic analysis reveals intraspecies diversification of antiviral receptor transporter proteins in Xenopus laevis.

摘要: The Receptor Transporter Protein (RTP) family is present in most, if not all jawed vertebrates. Most of our knowledge this protein comes from studies on mammalian RTPs, which are multi-function proteins that regulate cell-surface G-protein coupled receptor levels, influence olfactory system development, immune signaling, and directly inhibit viral infection. However, mammals comprise less than one-tenth extant vertebrate species, about the expression, function, evolution non-mammalian RTPs limited. Here, we explore evolutionary history We identify signatures positive selection many RTP clades characterize multiple, independent expansions outside what has been described mammals. find a striking expansion African clawed frog, Xenopus laevis, with 11 species as opposed to 1 4 most other species. RNA sequencing revealed X. laevis upregulated following stimulation. In functional assays, demonstrate at least three these infection by viruses, suggesting homologs may serve antiviral effectors Mammalia.