Quantitative metabolic network profiling of Escherichia coli: An overview of analytical methods for measurement of intracellular metabolites
作者： Yang Shen , Tohidi Fatemeh , Leihan Tang , Zongwei Cai , None
DOI: 10.1016/J.TRAC.2015.07.006
关键词:
摘要: Understanding the mechanism of metabolic network response to extracellular changes has become one primary objectives systems biology. Quantitative profiling model organisms such as E. coli is principal approach. Well-designed analytical protocols for determination intracellular metabolites may provide a crucial premise quantitative cellular metabolism. This article reviews and evaluates existing methods determining in terms sample preparation mass spectrometry (MS) analysis. Critical issues including variation status interference external pools during cell sampling, loss quenching procedures, degradation efficiency extraction, matrix effects separation quantification are discussed. Moreover, suggestions provided solve problems based on results from recent studies order establish precise reliable platform metabolites.
sci-hub.se 参考文章(99)
Metabolomic strategy for the classification and quality control of phytomedicine: a case study of chamomile flower (Matricaria recutita L.). Planta Medica. ,vol. 70, pp. 250- 255 ,(2004) , 10.1055/S-2004-815543
Martin Robert, Tomoyoshi Soga, Masaru Tomita, E. coli metabolomics: Capturing the complexity of a "simple" model Springer, Berlin, Heidelberg. pp. 189- 234 ,(2007) , 10.1007/4735_2007_0221
J. Monod, Thetechnique of continuous culture. Ann. Inst. Pasteur. ,vol. 79, pp. 390- 410 ,(1950)
Samantha Kirk, Claudio A. Avignone-Rossa, Michael E. Bushell, Growth limiting substrate affects antibiotic production and associated metabolic fluxes in Streptomyces clavuligerus Biotechnology Letters. ,vol. 22, pp. 1803- 1809 ,(2000) , 10.1023/A:1005670603596
O.H. Lowry, J. Carter, J.B. Ward, Luis Glaser, The Effect of Carbon and Nitrogen Sources on the Level of Metabolic Intermediates in Escherichia coli Journal of Biological Chemistry. ,vol. 246, pp. 6511- 6521 ,(1971) , 10.1016/S0021-9258(19)34144-4
Eliane Fischer, Uwe Sauer, Metabolic flux profiling of Escherichia coli mutants in central carbon metabolism using GC‐MS FEBS Journal. ,vol. 270, pp. 880- 891 ,(2003) , 10.1046/J.1432-1033.2003.03448.X
Bernd Moritz, Katharina Striegel, Albert A. de Graaf, Hermann Sahm, Kinetic properties of the glucose-6-phosphate and 6 phosphogluconate dehydrogenases from Corynebacterium glutamicum and their application for predicting pentose phosphate pathway flux in vivo European Journal of Biochemistry. ,vol. 267, pp. 3442- 3452 ,(2000) , 10.1046/J.1432-1327.2000.01354.X
SG Villas-Boas, J Nielsen, J Smedsgaard, MAE Hansen, U Roessner-Tunali, None, Metabolome Analysis: An Introduction ,(2007)
Moselio Schaechter, John L. Ingraham, Frederick C. Neidhardt, Physiology of the bacterial cell : a molecular approach Sinauer Associates. ,(1990)
Jae-Gu Pan, Seong Eon Ryu, Jung Ae Kim, Byung Jo Yu, Jeong Hee Moon, The diversity of lysine-acetylated proteins in Escherichia coli. Journal of Microbiology and Biotechnology. ,vol. 18, pp. 1529- 1536 ,(2008)