Kallikrein-mediated proteolysis regulates the antimicrobial effects of cathelicidins in skin
作者： Kenshi Yamasaki , Jürgen Schauber , Alvin Coda , Henry Lin , Robert A. Dorschner
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摘要: The presence of cathelicidin antimicrobial peptides provides an important mechanism for prevention infection against a wide variety microbial pathogens. activity is controlled by enzymatic processing the proform (hCAP18 in humans) to mature peptide (LL-37 human neutrophils). In this study, elements skin were examined. Unique distinct from LL-37 identified normal skin. Through use selective inhibitors, SELDI-TOF-MS, Western blot, and siRNA, serine proteases stratum corneum tryptic enzyme (SCTE, kallikrein 5) chymotryptic protease (SCCE, 7) shown control activation precursor protein hCAP18 also influence further smaller with alternate biological activity. importance vivo was illustrated SPINK5-deficient mice that lack inhibitor LEKTI. Epidermal extracts these animals show significant increase compared controls, immunoabsorption diminished These observations demonstrate balance proteolytic at epithelial interface will innate immune defense.
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