Lost expression of DCC gene in ovarian cancer and its inhibition in ovarian cancer cells

摘要: Ovarian cancer is a leading cause of cancer-related women mortality in China. In recent years, the molecular mechanisms involved ovarian carcinoma development and/or progression have been intensely studied, and several genes identified. Deleted Colorectal Carcinoma (DCC), an important tumor suppressor gene, which inactivated many kinds tumors, its function(s) not clarified. Even though lost expression DCC occurred later stages multistep colorectal carcinogenesis, contribution to onset or fully understood. To investigate cancer, we studied 254 clinical samples by RT–PCR. Our results revealed that 52% malignant did express gene. By contrast, was observed all normal ovary tissues 80% benign tumors. Obviously, there significant correlation between especially epithelial cancer. The present study also suggested loss more frequently cases stage, higher pathological grade, poorer prognosis. other part this study, further explored after transfection two caner cell lines, namely SKOV3 HO-8910 cell, using RT–PCR immunocytochemistry. indicated expressed SKOV3-DCC HO-8910-DCC cells, ultrastructural analysis showed appearance apoptotic features them. Furthermore, growth markedly down-regulated above groups indicating with constructs can suppress cells. conclusion, our suggest association gene may inhibit However, result needs trials larger sample.