An Isocratic UV HPLC Assay for Analysis of Total and Free Melphalan Concentrations in Human Plasma

摘要: Summary A simple isocratic HPLC-UV assay for measurement of total and free melphalan concentrations in human plasma is described. Samples were prepared by methanol precipitation (total assay) ultrafiltration (free assay). On a 25 cm × 4.6 mm C18 column with 0.016 m mixed soldium phosphate citrate buffer (pH 3.75)-acetonitrile 87:13 as mobile phase, at flow rate 1 mL min−1, the retention time was 11.5 min. Detection 254 nm. For response linear function concentration up to 40 μg mL−1, excellent interday precision (<6% 0.5–40 mL−1 melphalan), accuracy (<2% deviation from true concentration), recovery (91–110%). 2.5 good (<11% 0.7–2.5 melphalan) (89–93%). limits 0.1 0.05 assays, respectively. The assays clinically app...