M2 isoform of pyruvate kinase (PKM2) is upregulated in Kazakh’s ESCC and promotes proliferation and migration of ESCC cells
作者： Qing Liu , Meng Liang , Tao Liu , Lucine Vuitton , Shutao Zheng
DOI: 10.1007/S13277-015-4073-Z
关键词:
摘要: The objectives of the present study are to explore role pyruvate kinase isoenzyme type M2 (PKM2) in progression Kazakh's esophageal squamous cell carcinoma (ESCC) Xinjiang, China, and clarify mechanism PKM2 malignant phenotype. expression was examined using immunohistochemistry (IHC) 101 matched pairs ESCC normal adjacent tissues (NATs) enzyme-linked immunosorbent assay (ELISA) 35 serum samples 8 healthy subjects. To investigate mechanism, small interfering RNA (siRNA)-PKM2 transfected into cells. Cell migration invasion were evaluated by wound healing Transwell assays. Apoptosis cycle analyzed flow cytometry (FCM). significantly higher (77.2 %, 78/101) compared with NAT (P = 0.003) also patients (78.84 ng/mL) subjects (13.55 ng/mL) (P = 0.001). Patients overexpression had a poor prognosis (P = 0.032). After knockdown PKM2, proliferation, migration, reduced (P = 0.001), apoptosis increased arrested at G1 phase. correlated worse outcome ESCC. Furthermore, involved promoting proliferation suppressing apoptosis, accelerating invasion, influencing cycle. could be potential biomarker for molecular classification
springer.com
sci-hub.se 参考文章(30)
Marcellus Simadibrata, Murdani Abdullah, Ari Fahrial Syam, Achmad Fauzi, Abdul Aziz Rani, The value of fecal tumor M2 pyruvate kinase as a diagnostic tool for colorectal cancer screening. Acta medica Indonesiana. ,vol. 44, pp. 94- 99 ,(2012)
C Akrivakis, R Geppert, J Mesterharm, K Possinger, K D Wernecke, D Lüftner, P E Petrides, Tumor type M2 pyruvate kinase expression in advanced breast cancer Anticancer Research. ,vol. 20, pp. 5077- 5082 ,(2000)
Sarah B Umar, David E Fleischer, Esophageal cancer: epidemiology, pathogenesis and prevention Nature Clinical Practice Gastroenterology & Hepatology. ,vol. 5, pp. 517- 526 ,(2008) , 10.1038/NCPGASTHEP1223
Jae Yun Lim, Overexpression of the M2 isoform of pyruvate kinase is an adverse prognostic factor for signet ring cell gastric cancer World Journal of Gastroenterology. ,vol. 18, pp. 4037- 4043 ,(2012) , 10.3748/WJG.V18.I30.4037
R. Lambert, P. Hainaut, Epidemiology of oesophagogastric cancer Best Practice & Research in Clinical Gastroenterology. ,vol. 21, pp. 921- 945 ,(2007) , 10.1016/J.BPG.2007.10.001
Niki Karachaliou, Chara Papadaki, Eleni Lagoudaki, Maria Trypaki, Maria Sfakianaki, Anastasios Koutsopoulos, Dimitris Mavroudis, Efstathios Stathopoulos, Vassilis Georgoulias, John Souglakos, None, Predictive value of BRCA1, ERCC1, ATP7B, PKM2, TOPOI, TOPΟ-IIA, TOPOIIB and C-MYC genes in patients with small cell lung cancer (SCLC) who received first line therapy with cisplatin and etoposide. PLOS ONE. ,vol. 8, ,(2013) , 10.1371/JOURNAL.PONE.0074611
ShuTao Zheng, Lucine Vuitton, Ilyar Sheyhidin, Dominique Angèle Vuitton, YueMing Zhang, XiaoMei Lu, Northwestern China: a place to learn more on oesophageal cancer. Part one: behavioural and environmental risk factors. European Journal of Gastroenterology & Hepatology. ,vol. 22, pp. 917- 925 ,(2010) , 10.1097/MEG.0B013E3283313D8B
Yi-Jun Qi, Wei-Xia Chao, Jen-Fu Chiu, An overview of esophageal squamous cell carcinoma proteomics Journal of Proteomics. ,vol. 75, pp. 3129- 3137 ,(2012) , 10.1016/J.JPROT.2012.04.025
Heather R. Christofk, Matthew G. Vander Heiden, Marian H. Harris, Arvind Ramanathan, Robert E. Gerszten, Ru Wei, Mark D. Fleming, Stuart L. Schreiber, Lewis C. Cantley, The M2 splice isoform of pyruvate kinase is important for cancer metabolism and tumour growth Nature. ,vol. 452, pp. 230- 233 ,(2008) , 10.1038/NATURE06734
Wenhao Guo, Yu Zhang, Ting Chen, Yongsheng Wang, Jianxin Xue, Yonggang Zhang, Wenjing Xiao, Xianming Mo, You Lu, Efficacy of RNAi targeting of pyruvate kinase M2 combined with cisplatin in a lung cancer model Journal of Cancer Research and Clinical Oncology. ,vol. 137, pp. 65- 72 ,(2011) , 10.1007/S00432-010-0860-5