The post-translational trimethylation of diphthamide studied in vitro.
作者： J M Moehring , T J Moehring
DOI: 10.1016/S0021-9258(18)69001-5
关键词:
摘要: The amino acid diphthamide is a complex post-translational derivative of histidine that exists in eukaryotic and Archaebacterial elongation factor 2 (EF-2). Diphtheria toxin Pseudomonas exotoxin A catalyze the transfer an ADP-ribose residue from NAD to diphthamide, causing inactivation EF-2. We have used cytosolic extracts mutant CHO-K1 cells study biosynthesis vitro. identified chromatographically precursor form one complementation group documented addition 3 methyl residues S-adenosylmethionine this precursor. presence methyltransferase capable carrying out reaction vitro 15 diverse species.
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