Plasmin Inactivation with Pulsed Electric Fields
作者： HUMBERTO VEGA-MERCADO , JOSEPH R. POWERS , GUSTAVO V. BARBOSA-CÁNOVAS , BARRY G. SWANSON
DOI: 10.1111/J.1365-2621.1995.TB06310.X
关键词:
摘要: Plasmin (fibrinolysin E.C.3.4.21.7), an indigenous enzyme in bovine milk, added to simulated milk ultrafiltrate (SMUF) at 100 μg/mL (pH 6.11 and ionic strength 0.056 M) was treated 10°C 15°C with pulsed electric fields (HVPEF) of 15, 30 45 kV/cm number pulses 10, 20, 30, 40 50. The plasmin activity measured using a commercial assay, reduced 90% after 50 both processing temperature 15°C. Similar inactivation obtained when (100 μg/mL) SMUF heated 40°C for 15 min. Inactivation the depended on applied during treatment, intensity field, temperature.
sci-hub.se 参考文章(18)
S. E. Gilliland, M. L. Speck, Mechanism of the Bactericidal Action Produced by Electrohydraulic Shock1 Applied Microbiology. ,vol. 15, pp. 1038- 1044 ,(1967) , 10.1128/AM.15.5.1038-1044.1967
Baldwin Robertson, R. Dean Astumian, Frequency dependence of catalyzed reactions in a weak oscillating field The Journal of Chemical Physics. ,vol. 94, pp. 7414- 7419 ,(1991) , 10.1063/1.460173
I. Politis, K.F. Ng Kwai Hang, R.N. Giroux, Environmental factors affecting plasmin activity in milk. Journal of Dairy Science. ,vol. 72, pp. 1713- 1718 ,(1989) , 10.3168/JDS.S0022-0302(89)79286-9
Eric D. Bastian, Karlo G. Hansen, Rodney J. Brown, Inhibition of Plasmin by β-Lactoglobulin Using Casein and a Synthetic Substrate Journal of Dairy Science. ,vol. 76, pp. 3354- 3361 ,(1993) , 10.3168/JDS.S0022-0302(93)77673-0
M. Korycha-Dahl, B.Ribadeau Dumas, N. Chene, J. Martal, Plasmin Activity in Milk Journal of Dairy Science. ,vol. 66, pp. 704- 711 ,(1983) , 10.3168/JDS.S0022-0302(83)81848-7
K.L. Kohlmann, S.S. Nielsen, M.R. Ladisch, Effects of a Low Concentration of Added Plasmin on Ultra-High Temperature Processed Milk Journal of Dairy Science. ,vol. 74, pp. 1151- 1156 ,(1991) , 10.3168/JDS.S0022-0302(91)78267-2
A. Mizuno, Y. Hori, Destruction of living cells by pulsed high-voltage application IEEE Transactions on Industry Applications. ,vol. 24, pp. 387- 394 ,(1988) , 10.1109/28.2886
Eric D. Bastian, Rodney J. Brown, C. Anthon Ernstrom, Plasmin Activity and Milk Coagulation Journal of Dairy Science. ,vol. 74, pp. 3677- 3685 ,(1991) , 10.3168/JDS.S0022-0302(91)78557-3
A SALE, W HAMILTON, Effects of high electric fields on microorganisms: I. Killing of bacteria and yeasts Biochimica et Biophysica Acta. ,vol. 148, pp. 781- 788 ,(1967) , 10.1016/0304-4165(67)90052-9
Gérard Humbert, Charles Alais, The milk proteinase system Journal of Dairy Research. ,vol. 46, pp. 559- 571 ,(1979) , 10.1017/S002202990001760X