Binding of the immunomodulatory drug Bz-423 to mitochondrial FoF1-ATP synthase in living cells by FRET acceptor photobleaching
作者： Gary D. Glick , Michael Börsch , Anthony W. Opipari , Peter Graber , Jan Petersen
DOI: 10.1117/12.2209645
关键词:
摘要: Bz-423 is a promising new drug for treatment of autoimmune diseases. This small molecule binds to subunit OSCP the mitochondrial enzyme FoF1-ATP synthase and modulates its catalytic activities. We investigate binding mitochondria in living cells how rotation synthase, i.e. mechanochemical mechanism this enzyme, affected by Bz-423. Therefore, was marked selectively genetic fusion with fluorescent protein EGFP C terminus gamma. Imaging threedimensional arrangement yeast possible at superresolution using structured illumination microscopy, SIM. measured uptake Cy5-labeled derivative FRET acceptor photobleaching microscopy. Our data confirmed top F1 domain Saccharomyces cerevisiae cells.
arxiv.org
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