Proteomic comparison of Ralstonia solanacearum strains reveals temperature dependent virulence factors.

摘要: Ralstonia solanacearum, the causal agent of bacterial wilt, is a genetically diverse plant pathogen present in tropical and subtropical regions world that infects more than 200 species, including economically important solanaceous crops. Most strains R. solanacearum are only pathogenic at temperatures between 25 to 30°C with can cause disease below 20°C considered threat agriculture temperate areas. Identifying key molecular factors distinguish virulent cold from ones not needed develop effective management tools for this pathogen. We compared protein profiles two low temperature when incubated rhizosphere tomato seedlings 30 18°C using quantitative 2D DIGE gel methods. Spot intensities were quantified compared, differentially expressed proteins sequenced identified by mass spectrometry (MS/MS). Four hundred eighteen (418) spots produced 101 unique proteins. The classified Gene Ontology biological processes categories metabolism, cell processes, stress response, transport, secretion, motility, virulence. Identified virulence included catalase (KatE), exoglucanase A (ChbA), drug efflux pump, twitching motility porin (PilQ). Other components putative type VI secretion system. confirmed differential expression 13 candidate genes real time PCR techniques. Global regulators HrpB HrpG also had dependent PCR. involvement discussed. discovery functional system provides new potential mechanism explore. global HrpB, suggest be partially explained differences regulation all strains.