hCLE/C14orf166 Associates with DDX1-HSPC117-FAM98B in a Novel Transcription-Dependent Shuttling RNA-Transporting Complex
作者： Alicia Perez-Gonzalez , Alejandra Pazo , Rosana Navajas , Sergio Ciordia , Ariel Rodriguez-Frandsen
DOI: 10.1371/JOURNAL.PONE.0090957
关键词:
摘要: hCLE/C14orf166 is a nuclear and cytoplasmic protein that interacts with the RNAP II, modulates RNA metabolism present in granules involved localized translation. Here we have studied whether hCLE shares common interactors nucleus cytosol, which could shed light on its participation sequential phases of metabolism. Nuclear purified hCLE-associated factors were identified proteins mRNA metabolism, motor-related proteins, cytoskeletal translation-related found. Purified complexes also contain RNAs as expected some hCLE-interacting (DDX1, HSPC117, FAM98B) found both cytoplasm. Moreover, endogenous fractionates together DDX1, HSPC117 FAM98B silencing down-regulates their cytosolic accumulation levels. Using photoactivatable hCLE-GFP protein, import export was observed indicating shuttling protein. Interestingly, required active transcription, did proteins. The data indicate probably complex shuttles between cytoplasm transporting suggesting this has prominent role fate.
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