Radioautographic study of glycoprotein biosynthesis and renewal in the ovarian follicles of mice and the origin of the zona pellucida.

摘要: L-fucose-3H was injected intravenously into mice which were killed at several time intervals after injection and semi-thin sections of their ovaries processed for radioautography analysed quantitatively. At the same specific activity serum glycoproteins determined. Glycoprotein biosynthesis demonstrated in oocytes, granulosa stromal cells. The silver grain density follicular fluid large follicles reached a peak 4 h, remained high 8 h decreased steadily subsequent intervals. It that labeling pattern depends on secretory cells also glycoproteins. collapsed zonae pellucidae represent highest degree atresia are able to take up glycoprotein macromolecules. Based this finding it shown there is very little synthesis zona pellucida follicles. A more occurred medium Following growth these having previously labeled pellucida, extracellular structure secreted by oocyte.