作者: Jiafeng Li , Junsheng Lou , Guodong Bao , Chenyu Wu , Zhiling Lou
DOI: 10.2139/SSRN.3556620
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摘要: Background: Random skin flaps are widely applied to rebuild and restore soft-tissue damage in reconstructive surgery. Nevertheless, severe ischemia ischemia/reperfusion injury lead flap necrosis, a major complication after operation occurring Exenatide exert therapeutic role diabetic wounds, cardiac injury, nonalcoholic fatty liver disease. However, whether has protective effect against necrosis remains unknown, the underlying mechanism should be explored further. Methods: One hundred forty-four mice were stochastically divided into Control (n=30), Exe(n=30), Exe + 3 methyladenine (3MA) (n=24), chloroquine (CQ) Scramble control (n=18), TFE3 shRNA groups (n = 18). Flap tissues obtained on postoperative day 7 evaluate tissue vitality, angiogenesis, pyroptosis, oxidative stress, autophagy using Immunohistochemistry, Immunofluorescence staining western blotting. Findings: We found increased enhanced reduced stress alleviated pyroptosis activate random flap. Inhibition of by 3MA CQ reversed above effects flap, suggesting that pro-survival In mechanistic investigation, activation nuclear translocation was mediate – induced autophagy. Besides, may result from AMPK-SPK2-CARM1 AMPK-mTOR signaling axis. Interpretation: Our findings revealed boosted viability, via upregulation These indicated is potent enhancer capable for promoting viability providing promising novel strategy. Funding Statement: Financial supports Public Welfare Technology Research Project Natural Science Foundation China (No. 81601705 Kailiang Zhou, No.81873942 Weiyang Gao, No. 81801930 Jian Ding, 81572227 81873992 Huazi Xu); Zhejiang Provincial Medicine Health 2017KY472 Zhou); Wenzhou Bureau (No.2016Y0350 Ding). Declaration Interests: The author states there no conflict interest. Ethics Approval Experimental steps involving animals strictly abided Guide Care Use Laboratory Animals National Institutes Health, which approved Animal Committee Medical University (wydw2017–0022).