ニホンカモシカ(Capricornis crispus)の糞中DNA解析による雌雄判別
作者： Takashi NISHIMURA , Kiyoshi YAMAUCHI , Yasushi SAITOH , Yoshitaka DEGUCHI , Toshiki AOI
DOI: 10.5686/JJZWM.15.73
关键词:
摘要: In this study we extracted DNA from feces of the Japanese serow and developed a PCR-based sex identification method using amelogenin gene. Fecal was by phenol chloroform with purification CTAB method. Analysis PCR products showed clear female male banding patterns one homozygous band (AMEL XX) 2 or 3 heterozygous bands XY), respectively, lengths approximately 250 bp 200-300 bp, electrophoresis on 2% agarose gel. Determination possible for fecal samples collected rectum serows in 38 40 individuals (95%), designations were completely consistent determination physical examination. successful 5 6 masses (83.3%) Takizawa Forest, judged to be origin origin. As sexual dimorphism is not remarkable, can used estimate based field analysis.
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