Introduction of β-d-mannuronic acid (M2000) as a novel NSAID with immunosuppressive property based on COX-1/COX-2 activity and gene expression.

摘要: Abstract Background The NSAIDs which inhibit the cyclooxygenase (COX) enzymes are among medications widely used to treat pain and inflammation. These drugs cause digestive complications resulting in inhibition of COX-1 enzyme, while COX-2 enzyme has therapeutic effects. Therefore research focuses on production that specifically enzyme. This study aimed effects β- d -mannuronic (M2000) acid gene expression activity COX-1/COX-2 order introduce a novel NSAID for treating inflammatory diseases. Methods mRNA levels COXs were analyzed with qRT-PCR. Prostaglandin E2 (PGE2) concentration culture media was determined using ELISA method. Results Our results indicated M2000 at low high dose could significantly reduce level compared LPS group (p  Conclusions showed drug as immunosuppressive property is able strongly enzymes, suppressing specifically. Therefore, based findings this might be categorized introduced selective inhibitory effect.