Dynamic changes in the CD163+ and CCR2+ peripheral monocytes during Parkinson’s disease

摘要: Background: Alpha-synuclein aggregates and accumulation are associated with immune activation neurodegeneration in Parkinson9s disease. The is not only dependent on the brain-resident microglial cells but also involves peripheral cells, such as mononuclear phagocytes including monocytes dendritic found blood well infiltrated into brain. Understanding involvement of component disease essential for development immunomodulatory treatment, which might modify progression. We aimed to study profile circulating early- late-stage by analyzing surface-expressed molecules related phagocytosis, alpha-synuclein sensing, tissue-migration. Methods: Multi-color flow cytometry from cross-sectional samples 80 gender-balance individuals sporadic disease, 29 controls, longitudinal seven patients one control. Cells were delineated natural killer monocyte subtypes, cell frequencies surface marker expressions compared between correlated standardized clinical motor non-motor scores. Results: Overall, we elevated levels markers migration (CCR2, CD11b) phagocytosis (CD163) particularly classical intermediate less than five years. This corresponded a decrease non-classical cells. observed an increased HLA-DR expression late sexual-dimorphism TLR-4 decreased women PD males. disease-associated changes TLR4, CCR2, CD11b symptoms olfaction or cognition. While many alterations normalized at stage, other remained, CD163 expressions. Conclusions: Our data highlight role CD163+ migration-competent further suggests that system dynamically altered stages directly both sex-bias