Comparison of the ability of enteroinvasive Escherichia coli, Salmonella typhimurium, Yersinia pseudotuberculosis, and Yersinia enterocolitica to enter and replicate within HEp-2 cells.

摘要: Salmonella typhimurium, enteroinvasive Escherichia coli, Yersinia pseudotuberculosis, and enterocolitica possess the ability to enter intestinal epithelial cells. We used a quantitative tissue culture model employing HEp-2 cells compare abilities of these bacteria S. typhimurium species were highly infective for but unable replicate extensively intracellularly. Enteroinvasive E. coli exhibited low infectivity replicated The growth led destruction monolayer, whereas spp. maintained intracellularly prolonged periods without damage monolayer. required prior at 37 degrees C; neither nor this temperature dependence cell entry. An K-12 derivative containing 230-kilobase plasmid from was constructed. This shared all invasive characteristics parental strain, suggesting that determinants entry intracellular multiplication least partially encoded. HB101 cloned invasion determinant Y. pseudotuberculosis constructed in our laboratory. monolayers coinfected with two derivatives those common genetic background. Results experiments suggest organisms reside within separate compartments.