A mutation in the semaphorin signalling pathway and its consequences in prostate cancer

摘要: Prostate cancer kills over 10,000 men every year in the UK, mainly as a consequence of spread disease to other sites body. It is important understand what controls prostate cells, this may lead treatments that either slow down or prevent metastasis, and perhaps result cure some advanced cancers. Semaphorins act chemotactic cues for axon guidance cell movement, via their transmembrane receptors, plexins. has been found semaphorins receptors are overexpressed human lines. These lines have screened mutations genes semaphorin signalling pathway. 13 somatic missense cytoplasmic domain Plexin B1 gene identified clinical samples primary metastatic cancers. One C5662T was predicted substitution Proline Serine. The aim research determine if mutation alters behaviour contributes progression. This thesis describes method constructing an expression vector containing transfecting into COS7 cells produce stable transient clones. The effect on collapse examined. possible show involved Rnd1 required process independent its ligand Semaphorin 4D (Sema4D). Although no functional difference between wild type mutant (C5662T) observed, rate be more work will needed define whether behaviour.