Streptococcus-Escherichia coli shuttle vector pSA3 and its use in the cloning of streptococcal genes.

摘要: Abstract A shuttle vector that can replicate in both Streptococcus spp. and Escherichia coli has been constructed by joining the E. plasmid pACYC184 (chloramphenicol tetracycline resistance) to streptococcal pGB305 (erythromycin resistance). The resulting chimeric is designated pSA3 (chloramphenicol, erythromycin, seven unique restriction sites: EcoRI, EcoRV, BamHI, SalI, XbaI, NruI, SphI. Molecular cloning into EcoRI or EcoRV site results inactivation of chloramphenicol resistance, SphI resistance coli. was transformed stable sanguis mutans presence erythromycin. We have used construct a library S. GS5 genome coli, expression surface antigens this heterologous host confirmed with antiserum. A previously cloned determinant specifies streptokinase subcloned pSA3, recombinant selective pressure expressed activity (Challis), mutans.