Diagnostic moléculaire par RT-PCR de la grippe équine au Maroc

摘要: Twenty-four nasopharyngeal swabs from suspect horses with equine influenza, taken the Casablanca region, were analyzed by RT-PCR (Reverse Transcription Polymerase Chain Reaction) to research for gene encoding matrix protein M of influenza virus. The aim this article is check specificity each methods: RT-PCRq (real time) and conventional (classic), in order confirm their correct application comparison analytical sensitivity. results sensitivity confirmed high detecting virus diluting at 5.10 .4 (viral charging: 150DCPE50/100μl), while classical revealed a detection limit dilution -3 Charging: 1,5.10 3 DCPE50/100µl). Due its sensitivity, was used test 24 suspected whose negative. However, simultaneous amplification sequence Beta-actin specific increases reliability our inhibition control.